In this verse, Jesus is preparing His disciples for the persecution they will face. He explains that those who oppose them do so because they do not truly know God the Father or Jesus Himself. This lack of knowledge isn't just about ignorance of facts; it's about a deeper, relational disconnect. Knowing God, in the biblical sense, implies a personal and intimate relationship, not just an awareness of His existence. Jesus emphasizes that many who act against His followers do so out of a misguided sense of duty or tradition, not realizing they are opposing God's work.
This statement is a call to believers to cultivate a genuine relationship with God, one that transforms their understanding and actions. It also encourages patience and compassion towards those who may oppose them, recognizing that their actions stem from a lack of true knowledge of God. By fostering a deeper connection with God, believers can better navigate challenges and misunderstandings, grounded in the love and truth of Christ.